Antigen

Antigen Class

Histone

Antigen

H3K27me3

Cell type

Cell type Class

Blood

Cell type

CD8+ T cells

NA

NA

Sample

source_name

T cells

strain

C57BL/6

tissue

spleen

cell type

CD8+ T cells

t cell subset

naïve

chip antibody

H3K27me3

chip antibody vendor

Upstate

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP-seq libraries were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model

Illumina Genome Analyzer

mm10

Number of total reads

13878001

Reads aligned (%)

98.6

Duplicates removed (%)

76.3

Number of peaks

255 (qval < 1E-05)

mm9

Number of total reads

13878001

Reads aligned (%)

98.4

Duplicates removed (%)

76.5

Number of peaks

282 (qval < 1E-05)