Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27me3

Cell type

Cell type Class
Blood
Cell type
CD8+ T cells
NA
NA

Attributes by original data submitter

Sample

source_name
T cells
strain
C57BL/6
tissue
spleen
cell type
CD8+ T cells
t cell subset
central memory (CM)
chip antibody
H3K27me3
chip antibody vendor
Upstate

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP-seq libraries were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model
Illumina Genome Analyzer

mm10

Number of total reads
15340241
Reads aligned (%)
98.7
Duplicates removed (%)
72.4
Number of peaks
95 (qval < 1E-05)

mm9

Number of total reads
15340241
Reads aligned (%)
98.6
Duplicates removed (%)
72.6
Number of peaks
95 (qval < 1E-05)

Base call quality data from DBCLS SRA