Antigen

Antigen Class

Bisulfite-Seq

Antigen

Bisulfite-Seq

Cell type

Cell type Class

Liver

Cell type

Liver

MeSH Description

A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.

Sample

source_name

Pemt+/+ under HFHS diet #75

genotype/variation

Pemt WT

tissue

Liver

diet

HFHS diet

Sequenced DNA Library

library_strategy

Bisulfite-Seq

library_source

GENOMIC

library_selection

RANDOM

library_construction_protocol

genomic DNA was extracted and purified from mouse liver samples using Qiagen DNeasy Kit according to standard instructions Bisulphite converted DNAs were enriched with EpiXplore Methylation DNA Enrichment Kit (Clontech). Genomic DNA libraries was prepared by TruSeq ChIP Sample Preparation Kit (Illumina).

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

93863403

Reads aligned (%)

94.0

Coverage rate (×)

0.5

Number of hyper MRs

104429 (qval < 1E-05)

mm9

Number of total reads

93863403

Reads aligned (%)

95.1

Coverage rate (×)

0.4

Number of hyper MRs

104388 (qval < 1E-05)