ChIP-Atlas: SRX9917770

Antigen

library_strategy: ChIP-Seq
library_source: GENOMIC
library_selection: ChIP
library_construction_protocol: ChIP-seq: Chromatin (8 μg) was precleared with protein A agarose beads (Invitrogen). Genomic DNA regions of interest were precipitated using 4 μg of antibody against H3K27Ac (Active Motif, Cat# 39133) or 10 μg of antibody again HAND1 (Abconal, Cat#A9855). The complexes were washed, eluted from the beads with 1% SDS buffer and subjected to RNase and proteinase K treatment overnight at 65°C. The ChIPed DNA was purified by phenol-chloroform extraction and ethanol precipitation. Genomic DNA (Input) was prepared by treating aliquots of chromatin with RNase, proteinase K in a similar way as ChIPed DNA. Illumina sequencing libraries were prepared from the ChIP products by the standard consecutive enzymatic steps of end-repair, A tailing, and adaptor ligation using the KAPA Hyper Prep Kit (Roche, CKK8504). Following a final PCR amplification step, the PCR products were purified and the size was selected by 0.5X SPRI followed by 0.9X SPRI beads.