Antigen

Antigen Class

TFs and others

Antigen

SMARCA4

Cell type

Cell type Class

Blood

Cell type

K-562

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Chronic Myelogenous

Sample

source_name

K562 cell line

cell line

K562

antibody

SMARCA4

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

K562 cells were cross-linked using 2 mM disuccinimidyl glutarate for 30min then in 1% formaldehyde for 10 min at room temperature. Chromatin was isolated using a ChIP-qPCR Kit (Chromatrap, cat. no. 500117) und sonicated using a Bioruptor Plus (Diagenode) to give a fragment distribution of 150-400 bp. ChIPed DNA was subjected library preparation using a NEBNext Ultra DNA Library Prep Kit for Illumina (NE Biolabs) following the manufacturer's protocol . Final products were sequenced by Illumina Nextseq 500 platform. For further details see methods section in the associated manuscript.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

66342128

Reads aligned (%)

87.3

Duplicates removed (%)

8.9

Number of peaks

14722 (qval < 1E-05)

hg19

Number of total reads

66342128

Reads aligned (%)

86.7

Duplicates removed (%)

9.9

Number of peaks

14871 (qval < 1E-05)