Antigen

Antigen Class

Bisulfite-Seq

Antigen

Bisulfite-Seq

Cell type

Cell type Class

Placenta

Cell type

Placenta

MeSH Description

A highly vascularized mammalian fetal-maternal organ and major site of transport of oxygen, nutrients, and fetal waste products. It includes a fetal portion (CHORIONIC VILLI) derived from TROPHOBLASTS and a maternal portion (DECIDUA) derived from the uterine ENDOMETRIUM. The placenta produces an array of steroid, protein and peptide hormones (PLACENTAL HORMONES).

Sample

source_name

placenta

developmental timepoint

full term birth

Sex

male

diagnosis

autism

Sequenced DNA Library

library_strategy

Bisulfite-Seq

library_source

GENOMIC

library_selection

RANDOM

library_construction_protocol

Samples of the placenta were frozen immediately after birth. DNA was purified using Qiagen's Puregene kit and sonicated to about 200 bp. Methylated adapters were added with the NEBNext DNA library prep kit. Libraries were bisulfite converted with the Zymo EZ DNA Methylation Lightning kit, amplified for 14 cycles with PfuTurbo Cx, and purified with AMPure beads.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

219194177

Reads aligned (%)

84.0

Coverage rate (×)

2.3

Number of hyper MRs

298378 (qval < 1E-05)

hg19

Number of total reads

219194177

Reads aligned (%)

84.4

Coverage rate (×)

2.6

Number of hyper MRs

286850 (qval < 1E-05)