Antigen

Antigen Class

TFs and others

Antigen

FLI1

Cell type

Cell type Class

Bone

Cell type

POE

NA

NA

Sample

source_name

Ewing sarcoma cell line

cell line

POE

condition

WT

antibody

FLI1

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP were done following manufacturer protocol using iDeal ChIP-seq kit for transcription factors and histones (Diagenode) with anti-HA , rabbit polyclonal anti-Fli, rabbit polyclonal anti-H3K4me3 and rabbit polyclonal anti-H3K27ac TruSeq Chip library preparation kit (Illumina)

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

20044834

Reads aligned (%)

98.5

Duplicates removed (%)

2.8

Number of peaks

2517 (qval < 1E-05)

hg19

Number of total reads

20044834

Reads aligned (%)

97.3

Duplicates removed (%)

3.8

Number of peaks

2478 (qval < 1E-05)