Antigen

Antigen Class

Histone

Antigen

H2AK119ub

Cell type

Cell type Class

Blood

Cell type

Hematopoietic Stem Cells

MeSH Description

Progenitor cells from which all blood cells derived. They are found primarily in the bone marrow and also in small numbers in the peripheral blood.

Sample

source_name

Lin- HSC

strain

C57BL/6

genotype/variation

R26CreERT2;Ring1a-/-;Ring1bf/f;Cdkn2a-/-

antibody

H2AK119 ubiquitination

antibody vendor/catalog/lot

Cell signaling D27C4/2

transduction

PML-RARa

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

The LIN- HSCs were isolated from the bone marrow of the indicated mice using the hematopoietic progenitor enrichment kit (StemCell Technologies), infected and kept in liquid culture Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit (Part# 0801-0303).

Sequencing Platform

instrument_model

Illumina Genome Analyzer II

mm10

Number of total reads

29690036

Reads aligned (%)

96.3

Duplicates removed (%)

6.6

Number of peaks

496 (qval < 1E-05)

mm9

Number of total reads

29690036

Reads aligned (%)

96.2

Duplicates removed (%)

6.7

Number of peaks

487 (qval < 1E-05)