GSM1644650: DMSO G9a 48hrs ChIP Rep2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
TFs and others
Cell type Class
Attributes by original data submitter
Sequenced DNA Library
Chromatin immunoprecipitation was performed using the ChIP-IT High Sensitivity kit (Active Motif #53040) according to manufacturer’s instructions except that Input samples were purified by phenol:chloroform extraction. The following antibodies were used: anti-H3K9me2 (Abcam #ab1220), anti-G9a (Abcam #ab40542), and Rabbit IgG (Jackson Immunoresearch #011-000-003). ChIP samples were quantitated using the Qubit 2.0 Fluorometer (Invitrogen). A portion of each sample was confirmed by qPCR. The remainder of the sample was submitted for high throughput sequencing. Libraries were prepared for high-throughput using the Illumina TruSeq kit as per manufacturer’s recommendations.