Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
KMT2D

Cell type

Cell type Class
Blood
Cell type
OCI-LY-7
Primary Tissue
Blood
Tissue Diagnosis
Lymphoma B-cell

Attributes by original data submitter

Sample

source_name
Lymphoma cell line
cell line
OCI-LY7
cell type
GCB-DLBCL
genotype
wt
stain
tumor
antibody
KMT2D antibody

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin was sheared to 200-400bp, incubated with KMT2D antibody (in-house generated human KMT2D antibody specific for the N-terminus previously described in Hu D. et. al Mol Cell Biol 2013 PMID:24081332) overnight at 4C on a rocker, antibody chromatin complex was captured by ProteinA agarose beads, washed with low salt, high salt, LiCl, and TE buffer, eluted and then de-crosslinked at 65C with 0.3M NaCl final for at least 6 hr. Final chormatin was purified using Qiagen PCR purification columns. KAPA HTP ChIP-seq sample prep kit (KAPA Bioystems)

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
21884340
Reads aligned (%)
87.4
Duplicates removed (%)
6.2
Number of peaks
29243 (qval < 1E-05)

hg38

Number of total reads
21884340
Reads aligned (%)
88.3
Duplicates removed (%)
5.7
Number of peaks
29272 (qval < 1E-05)

Base call quality data from DBCLS SRA