Antigen

Antigen Class

TFs and others

Antigen

Rorc

Cell type

Cell type Class

Blood

Cell type

Lymphoid organ

NA

NA

Sample

source_name

lymphoid organ

tissue

lymphoid organ

isolate

C57BL/6J

disease

NA

disease stage

NA

genotype

NA

chip antibody

polyclonal rabbit anti-RORgt antibodies (homemade)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated PFA-fixed nuclei, and Twin-strep-tagged RORa-DNA complexes were isolated with Strep-Tactin. ChIP-Seq libraries were prepared with the KAPA HyperPlus Kit (Roche) following the manufacturer's protocols.

Sequencing Platform

instrument_model

Illumina HiSeq 4000

mm10

Number of total reads

31877124

Reads aligned (%)

95.6

Duplicates removed (%)

10.1

Number of peaks

2970 (qval < 1E-05)

mm9

Number of total reads

31877124

Reads aligned (%)

95.4

Duplicates removed (%)

10.2

Number of peaks

3011 (qval < 1E-05)