GSM4973759: ERRa ChIPseq 2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
ESRRA
Cell type
Cell type Class
Breast
Cell type
MDA-MB-231
Primary Tissue
Breast
Site of Extraction
Effusion, Pleural
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
MDA-MB-231 cells
cell line
MDA-MB-231 breast cancer cells
tissue
breast cancer
chip antibody
ERRα: GTX108166, Genetex
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
10x10e6 cells were cross-linked with 1% formaldehyde and quenched for 5 min in 1 M Glycine. After centrifugation, cell pellets were resuspended in lysis buffer (1% SDS, 50 mM Tris-HCl pH8, 10 mM EDTA). Sonication was performed with Ultrasonicator (Covaris). Lysates from 2.5 x106 cells were processed with the iDEAL ChIP kit (Diagenode) according to the manufacturer's recommendations using 5 μg of antibody (ERRα: GTX108166, Genetex). Quality of samples was assessed on the Agilent TapeStation 4150 using D5000 ScreenTape and Agilent TapeStation Software 3.2. Libraries were prepared according to Illumina's instructions. The ChIP DNA libraries were sequenced as paired-ended 81bp reads with an Illumina Next Seq 500 sequencer following the manufacturer's protocols. Sequencing gave 29.106 and 10.106 reads for ERRα and input samples respectively.