Antigen

Antigen Class

TFs and others

Antigen

NR3C1

Cell type

Cell type Class

Pluripotent stem cell

Cell type

iPSC derived hepatocytes

NA

NA

Sample

source_name

Hepatocyte

cell type

hiPSC-derived HLCs

chip antibody

GR

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Total RNA was extracted by using Qiagen RNeasey Mini kit. DNA was isolated using phenol/chloroform extraction and NaCl/EtOH precipitated with 20 μg glycogen carrier. Transposed DNA was purified using a QIAGEN MinElute Reaction Cleanup kit. Transposed fragments were subjected to library preparation following manufacturer protocol The H3K4me3 in-situ ChIA-PET was performed according to the previously described protocol (Li et al., 2017) RNA libraries were prepared for sequencing by Novogene (CA). ChIP-seq library was prepared for sequencing according to the amplification protocol from Illumina. ATAC-seq libraries were sequenced using paired-end, dual-index sequencing on a NextSeq 500 instrument The H3K4me3 in-situ ChIA-PET was performed according to the previously described protocol (Li et al., 2017)

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

37960890

Reads aligned (%)

96.8

Duplicates removed (%)

37.5

Number of peaks

11364 (qval < 1E-05)

hg19

Number of total reads

37960890

Reads aligned (%)

96.5

Duplicates removed (%)

38.0

Number of peaks

11378 (qval < 1E-05)