Antigen

Antigen Class

TFs and others

Antigen

NR3C1

Cell type

Cell type Class

Kidney

Cell type

293

Primary Tissue

Kidney

Tissue Diagnosis

Normal

Sample

source_name

Isogenic HEK293 cells, SUMOylation-defective GR (GR3KR), vehicle, GR ChIP

cell line

HEK293

cell type

human embryonal kidney cell line

stably expressing

SUMOylation-defective GR (GR3KR)

treatment

DMSO + EtOH

concentration

0.01% + 0.01%

time

24 h + 1 h

chip antibody

GR (Santa Cruz, sc-1003, lot # D1812)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For ChIP-seq, lysates were clarified from sonicated nuclei, and protein-DNA complexes were isolated with specified antibody. For ATAC-seq, 100 000 isolated nuclei were treated with 2.5 µl Nextera Tn5 Transposase from Nextera kit. For RNA-seq, RNA was extracted using Qiagen RNeasy Mini kit. For ChIP-seq, NEBNext Ultra II DNA Library Prep Kit; for ATAC-seq, Illumina Nextera DNA Library Prep Kit; for RNA-seq, NEBNext Poly(A) mRNA Magnetic Isolation Module kit and NEBNext Ultra II Directional RNA Library Prep kit.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

41288792

Reads aligned (%)

93.1

Duplicates removed (%)

3.7

Number of peaks

957 (qval < 1E-05)

hg19

Number of total reads

41288792

Reads aligned (%)

92.5

Duplicates removed (%)

4.8

Number of peaks

823 (qval < 1E-05)