Antigen

Antigen Class

TFs and others

Antigen

VSV-G

Cell type

Cell type Class

Digestive tract

Cell type

SW 480

Primary Tissue

unresolved

Tissue Diagnosis

unresolved

Sample

source_name

Colorectal cancer cells

chip antibody

VSV (V4888, Sigma)

treatment

16 hours doxycyclin

stably transfected vector

pRTR

cell line

SW480

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared for sequencing using standard Illumina protocols according to standard procedures of GATC, Konstanz.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

13245968

Reads aligned (%)

83.7

Duplicates removed (%)

2.1

Number of peaks

557 (qval < 1E-05)

hg19

Number of total reads

13245968

Reads aligned (%)

82.9

Duplicates removed (%)

3.2

Number of peaks

588 (qval < 1E-05)