Antigen

Antigen Class

Histone

Antigen

H3K4me1

Cell type

Cell type Class

Others

Cell type

Lenses, Intraocular

MeSH Description

An intraocular lens permanently implanted behind the IRIS and in front of the natural EYE LENS.

Sample

source_name

newborn lens, H3K4me1 ChIP

strain/background

CD-1

developmental stage

newborn

age

P1

tissue

lens

chip antibody

H3K4me1 (Abcam, ab8895, lot# GR61280-1)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP-seq: The E12.5 forebrain and newborn (P1) lenses of CD-1 mice (Charles River Laboratories) were dissected, tissue was freshly crosslinked and homogenized. Sonicated lysates were clarified and histone-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

16263932

Reads aligned (%)

97.6

Duplicates removed (%)

9.3

Number of peaks

137 (qval < 1E-05)

mm9

Number of total reads

16263932

Reads aligned (%)

97.4

Duplicates removed (%)

9.4

Number of peaks

150 (qval < 1E-05)