Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Blood

Cell type

Neutrophils

MeSH Description

Immature neutrophils.

Sample

source_name

Neutrophil

cell type

Neutrophil

condition

healthy

chip antibody

N/A

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin was fixed in 1% formaldehyde, sheared to 100-900bp using Micrococcal nucleasean (Cell Signalling Technologies Inc.) and ultrasonic sonicator (FB-705, Fisher Scientific), immunoprecipitated using the indicated antibodies, purified and sequenced using Illumina HiSeq 2500 as recommended by the manufacturer.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

32695750

Reads aligned (%)

95.3

Duplicates removed (%)

4.3

Number of peaks

764 (qval < 1E-05)

hg19

Number of total reads

32695750

Reads aligned (%)

94.3

Duplicates removed (%)

6.1

Number of peaks

747 (qval < 1E-05)