Antigen

Antigen Class

TFs and others

Antigen

MYCN

Cell type

Cell type Class

Muscle

Cell type

RH-4

NA

NA

Sample

source_name

RH4

cell line

RH4

diagnosis

rhabdomyosarcoma

treatment

7 days treatment with 250nM cis conformation (inactive) PROTAC compound (University of Dundee, Institute of Chemical and Structural Biology available at Boehringer Ingelheim (opnMe))

genotype

wild type

antibody

anti-MYCN, Thermo Fisher, MA1-170

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

After reverse crosslinking, ChIP DNA was purified using MinElute PCR Purification Kit (Qiagen). ChIPseq Library

Sequencing Platform

instrument_model

Illumina HiSeq 4000

hg38

Number of total reads

32188190

Reads aligned (%)

86.4

Duplicates removed (%)

32.4

Number of peaks

1016 (qval < 1E-05)

hg19

Number of total reads

32188190

Reads aligned (%)

85.7

Duplicates removed (%)

33.6

Number of peaks

1141 (qval < 1E-05)