Antigen

Antigen Class

TFs and others

Antigen

KMT2A

Cell type

Cell type Class

Others

Cell type

Spleen

MeSH Description

An encapsulated lymphatic organ through which venous blood filters.

Sample

source_name

human cells from the spleen of engrafted NSG mouse

treatment

primary human FL CD34+ cells were CRISPR-Cas9 edited to induce an MLL-AF4 chromosomal translocation and engrafted into an NSG mouse

cell type

human cells isolated from the spleen of engrafted NSG mouse

chip antibody

Bethyl/A300-086A

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were washed with PBS and fixed in either 1% FA for 10 minutes (histones) or 2mM DSG for 30 minutes and 1% FA for 30 minutes (other, non-histone). Cells were sonicated to give fragments of 100-300bp followed by IP. DNA libraries were made using the NEBnext ultra DNA library preparation kit for Illumina (Cat no. E7370).

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

24243551

Reads aligned (%)

70.6

Duplicates removed (%)

6.4

Number of peaks

22451 (qval < 1E-05)

hg19

Number of total reads

24243551

Reads aligned (%)

70.0

Duplicates removed (%)

6.5

Number of peaks

22286 (qval < 1E-05)