GSM4891305: ChIP-seq LAPC4 AR DHT rep1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
AR
Cell type
Cell type Class
Prostate
Cell type
LAPC-4
NA
NA
Attributes by original data submitter
Sample
source_name
ChIP-seq LAPC4 AR DHT
cell line
LAPC4
cell type
human prostate cancer cells
treatment
DHT
chip antibody
AR (Sigma, EMB Millipore, #06-680)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were crosslinked for 10 min with 1% formaldehyde and quenched with 125 mM glycine for 5 min. Cells were then washed with PBS, pelleted, flash frozen and stored at -80°C. Lysates were clarified from sonicated nuclei and AR- or KLF4-DNA complexes were isolated with antibody. Libraries were prepared according to NEB's instructions accompanying the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®. After adapter ligation, DNA was PCR amplified for 12 cycles. The purified DNA was captured on an S4 Flowcell (PE100) for cluster generation. Libraries were sequenced on the Illumina NovaSeq 6000 following the manufacturer's protocols at the next generation sequencing platform of Centre de Recherche du CHU de Québec (CRCHUQ).