Antigen

Antigen Class

TFs and others

Antigen

MYC

Cell type

Cell type Class

Bone

Cell type

U2OS

Tissue

bone

Lineage

mesoderm

Description

osteosarcoma from the tibia of a 15 year old, J. Ponten and E. Saksela derived this line (originally 2T) in 1964 from a moderately differentiated sarcoma, viruses were not detected during co-cultivation with WI-38 cells or in CF tests against SV40, RSV or adenoviruses, mycoplasma contamination was detected and eliminated in 1972, (PMID: 6081590)

Sample

source_name

ChIPseq_MYCER_MYC_30min_OHT

cell line

U2OS

cell type

osteosarcoma cell line

treatment

30' 4-OHT treament

chip antibody

c-Myc (Y69)(rabbit, abcam, ab32072)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

crosslink (5 min), harvest, 10% of NIH 3T3 spike in, lysis of cells, fragment with covaris libraries were prepared for sequencing using standard NEB protocols

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

16152354

Reads aligned (%)

91.8

Duplicates removed (%)

6.6

Number of peaks

15616 (qval < 1E-05)

hg19

Number of total reads

16152354

Reads aligned (%)

91.2

Duplicates removed (%)

8.1

Number of peaks

15550 (qval < 1E-05)