Antigen

Antigen Class

TFs and others

Antigen

ERF

Cell type

Cell type Class

Blood

Cell type

HUDEP-2

NA

NA

Sample

source_name

HUDEP-2

cell line

HUDEP-2

cell type

umbilical cord blood cells derived

genotype

ERF KO

chip antibody

ERF (ab153726, Abcam)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and ERF-DNA complexes were isolated with antibody. ChIP-seq libraries were prepared for sequencing using standard Illumina protocols.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

12856387

Reads aligned (%)

97.4

Duplicates removed (%)

18.8

Number of peaks

597 (qval < 1E-05)

hg19

Number of total reads

12856387

Reads aligned (%)

96.6

Duplicates removed (%)

19.1

Number of peaks

243 (qval < 1E-05)