Antigen

Antigen Class

TFs and others

Antigen

ING5

Cell type

Cell type Class

Liver

Cell type

Hep G2

Primary Tissue

Liver

Tissue Diagnosis

Carcinoma Hepatocellular

Sample

source_name

HepG2 cells

cell line

HepG2

tissue source

Liver

chip antibody

ING5 (Proteintech, 10665-1-AP)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. NG5-associated DNAs were amplified using non-biased conditions, labeled, and sequenced with HiSeq 2500 (Berry Genomics, Beijing).

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

20501704

Reads aligned (%)

81.8

Duplicates removed (%)

15.3

Number of peaks

1005 (qval < 1E-05)

hg19

Number of total reads

20501704

Reads aligned (%)

81.1

Duplicates removed (%)

15.8

Number of peaks

615 (qval < 1E-05)