GSM4851087: K120R NT (untreated); Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
TP53
Cell type
Cell type Class
Lung
Cell type
NCI-H1299
Primary Tissue
Lung
Tissue Diagnosis
Carcinoma Non-Small Cell
Attributes by original data submitter
Sample
source_name
H1299 cells
stable cell line
Tet Repressor expression
treatment
No treatment
antibody
anti-p53 (FL393-x, Santa Cruz)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cell lysates were clarified from sonicated nuclei and hp53-DNA complexes were isolated with p53-specific antibody. Library preparation was done using Swift's Biosciences Accel-NGS 2S Plus Library Kits for Illumina platforms. 10 ng of DNA was used for library preparation and kit's protocol was followed for a 200 base pair insert. An additional two sided bead clean up was done after the PCR step using SPRI beads with the formula .6X, then using the supernatant, 1.2x. The library was pooled to 1 nM and the sequenced on a NextSeq 500 using the standard loading protocol.