GSM4836893: PRDM1 hPGCLC r1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
MYC
Cell type
Cell type Class
Pluripotent stem cell
Cell type
PGCLC
NA
NA
Attributes by original data submitter
Sample
source_name
Human primordial germ cell-like cells
cell source
NANOS3–tdTomato hESCs bearing Dex-inducible SOX17 and Dox-inducible PRDM1 transgenes
cell type
Human primordial germ cell-like cells
chip antibody
Myc (Cell Signaling Technology, 2276)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
The whole day 2 embryoid bodies with hPGCLCs and day 2 DE cells (around 1.5-1.7 million cells) were collected for chromatin immunoprecipitation using the SimpleChIP Enzymatic Chromatin IP Kit (Magnetic Beads) (Cell Signaling Technology, 9003). Briefly, The cell pellets were washed twice with cold PBS containing 0.1% BSA and then fixed with paraformaldehyde. Following chromatin digestion with Micrococcal Nuclease, 2% volume of nuclei lysate was removed and stored at -80 °C as input control while the rest of the lysate was subjected to immunoprecipitation with anti-HA (Cell Signaling Technology, 3724) or anti-Myc (Cell Signaling Technology, 2276) antibody. After elution of chromatin, reversal of cross-links and DNA purification, the ChIP and input DNA were prepared for sequencing using the KAPA HyperPrep Kit following the manufacturer's instructions. Quantified and validated libraries were subjected to single-end or paired-end sequencing on HiSeq 4000 sequencing system (Illumina).