GSM4835936: BRD4 SAHA ChIP Rep2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
BRD4
Cell type
Cell type Class
Blood
Cell type
HL-60
Primary Tissue
Blood
Tissue Diagnosis
Leukemia
Attributes by original data submitter
Sample
source_name
HL60
cell line
leukemia cell line HL60
chip antibody
BRD4
treatment
SAHA
spike-in genome
dm3
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
RNA was extracted using TRIzol reagent. For H4ac ChIP, HL60 cells were cultured to a density of 7x10^5 cell/mL and treated with 1 µM SAHA or 0.01% DMSO for 1 h prior to ChIP protocol. Sequencing libraries were generated from DNA enriched by ChIP following the manufacturer's specification for the Tru-Seq library preparation kit (Illumina)