Antigen

Antigen Class

TFs and others

Antigen

NR3C1

Cell type

Cell type Class

Lung

Cell type

Lung cancer cell line

NA

NA

Sample

source_name

Lung cancer cell line

chip antibody

GR Ab

treatment

Vehicle

replicate

2

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin immunoprecipitations (ChIP) were performed as described (Stelloo et al. Oncogene 2018). For each ChIP 5 μg of GR antibody prebound to protein G beads were used. Immunoprecipitated DNA was processed for library preparation (Part# 0801-0303 KAPA biosystems kit).

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

25921777

Reads aligned (%)

98.6

Duplicates removed (%)

1.8

Number of peaks

824 (qval < 1E-05)

hg19

Number of total reads

25921777

Reads aligned (%)

97.9

Duplicates removed (%)

3.1

Number of peaks

893 (qval < 1E-05)