GSM4830083: Chip-seq IRF9 K562 + IFNa 6h; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
IRF9
Cell type
Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous
Attributes by original data submitter
Sample
source_name
Chip-seq IRF9 K562 + IFNa 6h
cell line
K562
cells used
10.00E+05
treatment
IFNa stimulation for 6 hours
antibody
Cell signaling (D2T8M)
antibody quantity
5 µg
magnetic beads
Protein G dynabeads
bead volume
100 µl
estimated fragment size
200
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Phenol-chroloform Library preparation was carried out as per manufacturer's instructions (Illumina) using 10ng material. Samples were subsequently size-selected via gel migration and extraction, with average cut sizes at about 230 bp, including adapters. Sequencing was carried out on an Illumina NextSeq 500. The quality of libraries was checked with the Bioanalyser DNA High sensitivity and by Q-PCR.