Antigen

Antigen Class

TFs and others

Antigen

BATF3

Cell type

Cell type Class

Blood

Cell type

Mac-1

NA

NA

Sample

source_name

peripheral blood

cell line

Mac-1

cell type

anaplastic large cell lymphoma cells

alk status

ALK−

chip antibody

BATF3

tissue

n.a.

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For each ChIP, 5 μg of antibody was conjugated to 2 mg of M-270 Epoxy Dynabeads (Thermo Fisher) and added to sonicated nuclear extract. Library construction was performed using the Swift 2S Turbo DNA Library Kit (Swift Biosciences) as per the manufacturer's instructions.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

42460152

Reads aligned (%)

97.9

Duplicates removed (%)

5.6

Number of peaks

23302 (qval < 1E-05)

hg19

Number of total reads

42460152

Reads aligned (%)

97.0

Duplicates removed (%)

6.8

Number of peaks

23039 (qval < 1E-05)