Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H2A.V

Cell type

Cell type Class
Adult
Cell type
Egg chamber
NA
NA

Attributes by original data submitter

Sample

source_name
H2Av[?CT] stage 10B follicle cell nuclei
strain background
OregonR
genotype/variation
w, P{His2Av[deltaCT], w+}; His2Av[810]/TM6B
developmental stage
10B
tissue
egg chamber

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Egg chambers were dounced and sonicated using a Bioruptor 300 (Diagenode). Libraries were constructed using the NEB Next ChIP seq kit (Part # E6200S) according to manufactures recommendations with indexing primers for multiplexing.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

dm6

Number of total reads
25903929
Reads aligned (%)
87.6
Duplicates removed (%)
46.0
Number of peaks
4805 (qval < 1E-05)

dm3

Number of total reads
25903929
Reads aligned (%)
87.7
Duplicates removed (%)
45.7
Number of peaks
4885 (qval < 1E-05)

Base call quality data from DBCLS SRA