Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Adult

Cell type

Egg chamber

NA

NA

Sample

source_name

OrR stage 10B follicle cell nuclei

strain background

OregonR

genotype/variation

wild type

developmental stage

10B

tissue

egg chamber

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Egg chambers were dounced and sonicated using a Bioruptor 300 (Diagenode). Libraries were constructed using the NEB Next ChIP seq kit (Part # E6200S) according to manufactures recommendations with indexing primers for multiplexing.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

dm6

Number of total reads

19327336

Reads aligned (%)

75.7

Duplicates removed (%)

51.5

Number of peaks

3938 (qval < 1E-05)

dm3

Number of total reads

19327336

Reads aligned (%)

75.9

Duplicates removed (%)

51.2

Number of peaks

3942 (qval < 1E-05)