GSM4790101: VCaP AR siSIM2 EtOH rep2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
AR
Cell type
Cell type Class
Prostate
Cell type
VCaP
Primary Tissue
Prostate
Tissue Diagnosis
Carcinoma
Attributes by original data submitter
Sample
source_name
VCaP prostate cancer cells, vehicle, siSIM2, AR ChIP
cell line
VCaP
cell type
Vertebral Cancer of the Prostate
sirna treatment
ON-TARGETplus SMARTpool against SIM2 (Dharmacon, L-008598-00, lot # 180323)
sirna concentration
30 nM
sirna time
72 h
hormone treatment
EtOH vehicle
hormone concentration
0.01%
hormone time
1 h
chip antibody
AR (rabbit antiserum; Karvonen et al. 1997)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP-seq, lysates were clarified from sonicated nuclei, and protein-DNA complexes were isolated with specified antibody. For ATAC-seq, 100 000 isolated nuclei were treated with 2.5 µl Nextera Tn5 Transposase from Nextera kit. For RNA-seq, RNA was extracted using Qiagen RNeasy Mini kit. For ChIP-seq, NEBNext Ultra II DNA Library Prep Kit; for ATAC-seq, Illumina Nextera DNA Library Prep Kit; for RNA-seq, NEBNext Poly(A) mRNA Magnetic Isolation Module kit and NEBNext Ultra II Directional RNA Library Prep kit.