GSM4779313: GGTI1 rep1 ChIPSeq; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
YAP1
Cell type
Cell type Class
Pluripotent stem cell
Cell type
iPSC derived endothelial cells
NA
NA
Attributes by original data submitter
Sample
source_name
hiPSC-ECs
cell type
hiPSC-ECs
treatment
GGTI298
antibody
YAP, Novus Biologicals, NB110-583582
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
hiPSC-ECs RNA was harvested using Trizol reagent. ATAC-seq DNA was extracted as described in previous protocol (Buenrostro J et al. Nat Methods 10: 1213-1218). Genomic DNA was clarified from sonicated nuclei and YAP-DNA complexes were isolated with antibody. RNA Libraries prepared from 10ng RNA, Sequencing data was un-stranded, ERCC spike-in (Ambion) used for data normalization. ATAC-seq libraries were prepared for sequencing using TruSeq Library Prep Kit (Illumina). ChIP-seq libaries were prepared following Zymo Spin-ChIP kit protocol from Zymo Research.