GSM4763931: MCF7 DMSO (replicate 1); Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
JUN
Cell type
Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
Breast carcinoma cell line
cell line
MCF7 breast carcinoma cell line
treatment
DMSO
treatment length
7 days
chip antibody
c-Jun (Cell Signaling Technologies, Cat# 9165)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were washed in phosphate-buffered saline (PBS), cross-linked with DSG for 45 minutes followed by fixation with 1% formaldehyde (methanol-free) for 10 minutes at room temperature, quenched with 0.125 M glycine for 5 min at room temperature and washed with PBS complete. Cells were resuspended in lysis buffer (1%SDS, 50 mM Tris-HCl pH8, 10 mM EDTA) containing protease and deacetylase inhibitors, and incubated on ice for 15 min. Lysed cells were sonicated for 5 minutes with a Covaris E220 sonicator (PIP140, DF5%, 200cpb), in 1ml AFA Fiber milliTUBEs. 5 µg of soluble chromatin were immunoprecipitated overnight with 10 μg of c-JUN antibody (CST 9165) on protein A and G magnetic beads; and finally washed, eluted and DNA purified. ChIP-seq libraries were constructed using Accel-NGS 2S DNA library kit from Swift Biosciences for c-Jun ChIP-seq.