Antigen

Antigen Class

TFs and others

Antigen

GATA1

Cell type

Cell type Class

Blood

Cell type

HUDEP-2

NA

NA

Sample

source_name

1151779_Hudep2_GATA1_rep2

cell line

Hudep2

antibody

GATA1

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Immunoprecipitated DNA was purified using the Qiagen PCR Extraction Kit and eluted with 20 µL of EB elution buffer. Sequencing libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit (NEB, cat. #E7645) with homemade TruSeq adaptors. Libraries were sequenced using an Illumina HiSeq 4000 system. NA

Sequencing Platform

instrument_model

Illumina HiSeq 4000

hg38

Number of total reads

67562959

Reads aligned (%)

95.0

Duplicates removed (%)

17.1

Number of peaks

7438 (qval < 1E-05)

hg19

Number of total reads

67562959

Reads aligned (%)

94.4

Duplicates removed (%)

18.2

Number of peaks

7293 (qval < 1E-05)