GSM4759258: MCF7 2 DMSO Fra2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
FOSL2
Cell type
Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
Breast carcinoma cell line
cell type
MCF7 breast carcinoma cell line
treatment
DMSO
treatment length
7 days
chip antibody
Fra2 (CST 19967)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP, cells crosslinked with two agents starting with 2 mM DSG (Pierce) for 45 minutes at room temperature followed by 1 mL of 1% formaldehyde for 10 minutes. Cross-linked cell lines or tissues were quenched with 0.125 M glycine for 5 minutes at room temperature. Cross-linked material was resuspended in 0.1% SDS (50 mM Tris-HCl pH8, 10 mM EDTA) and sonicated for 20 minutes with a Covaris S2.Thirty micrograms of soluble chromatin were immuoprecipiated with Protein A Dynabeads and 1 ?g of Fra2 (CST 19967) antibody. ChIP-seq libraries were constructed using NEBNextｮ UltraTM II kit from New England Biolabs.