Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
FLI1

Cell type

Cell type Class
Blood
Cell type
OCI-LY1
NA
NA

Attributes by original data submitter

Sample

source_name
OCILy1
cell line
OCILy1
cell type
lymphoma
cell of origin
GCB
chip antibody
FLI1, Rabbit polyclonal anti-FLI1 (ab-15289, Abcam)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and FLI1-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit (Part# 0801-0303). The purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the Illumina NextSeq 500 following the manufacturer's protocols.

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
1937
Reads aligned (%)
70.2
Duplicates removed (%)
1.6
Number of peaks
0 (qval < 1E-05)

hg19

Number of total reads
1937
Reads aligned (%)
70.0
Duplicates removed (%)
1.7
Number of peaks
0 (qval < 1E-05)

Base call quality data from DBCLS SRA