Antigen

Antigen Class

TFs and others

Antigen

USF2

Cell type

Cell type Class

Blood

Cell type

SEM

Primary Tissue

Blood

Tissue Diagnosis

Leukemia

Sample

source_name

B cell precursor leukemia

cell type

B cell precursor leukemia

cell line

SEM

tissue

B cell precursor leukemia

genotype/variation

SgUSF2.2

chip antibody

USF2 (NBP1-92649, Novus)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cut&Run ChiP-seq was performed according the the protocol in, Skene PJ, Henikoff S. 2017. An efficient targeted nuclease strategy for high-resolution mapping of DNA binding sites on Elife. Antibodies against USF2 for a pulldown assay. USF2 antibody from NBP1-92649, Novus was used for immunoprecipitation. NEBNext Ultra II DNA Library Prep Kit from New England Biolabs (E7645S)

Sequencing Platform

instrument_model

Illumina MiSeq

hg38

Number of total reads

9048829

Reads aligned (%)

82.2

Duplicates removed (%)

3.8

Number of peaks

3464 (qval < 1E-05)

hg19

Number of total reads

9048829

Reads aligned (%)

81.6

Duplicates removed (%)

4.0

Number of peaks

3433 (qval < 1E-05)