GSM4712420: ZBTB33/KAISO-ChIP; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
ZBTB33
Cell type
Cell type Class
Neural
Cell type
T98G
Primary Tissue
Brain
Tissue Diagnosis
Glioblastoma
Attributes by original data submitter
Sample
source_name
T98G
cell line
T98G
cell type
Brain-Glioblastoma
chip antibody
KAISO(6F8, Santa Cruz, sc-23871)
treatment
untreated
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Preparation of cross-linked chromatin free of RNA, sonication, and immunoprecipitation was performed as previously described (Di Vona et al., 2015). DNA was quantified with Qubit HS kit (Invitrogen). DNA libraries were generated by using the OvationÒ Ultralow Library System V2 (NuGEN) following the manufacturer's instructions. To minimize false positives calling, several input libraries were sequenced to reach saturation with a coverage of 4 reads/bp for the human genome for each condition.