GSM4710520: MDA231ERb E2+TNFa NFkB rep1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
NFKB1
Cell type
Cell type Class
Breast
Cell type
MDA-MB-231
Primary Tissue
Breast
Site of Extraction
Effusion, Pleural
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
Breast cancer cell line
cell line
MDA-MB-231-Erbeta
treatment
E2+TNFα
antibody
NFκB
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Antibodies include: H3K27me3 (Abcam ab 6002), H3K27ac (Abcam ab 4729), NFκB (Cell signaling 8242), RNA Pol II phospho Ser2 (Bethyl, A300-654A) ChIP-seq libraries were prepared from immunoprecipitated chromatin solutions and input DNA using the ThruPLEX® DNA-seq Kit V2 (Rubicon Genomics, Ann Arbor, MI).