Antigen

Antigen Class

TFs and others

Antigen

TFAP2A

Cell type

Cell type Class

Digestive tract

Cell type

Colorectal carcinoma

NA

NA

Sample

source_name

colorectal carcinoma (ATCC CCL-247)

modification

clone 24 with CRISPR/Cas9 KO of AP-2a

pasages

4

antibody

AP-2α 3B5 antibody (Santa Cruz, catalog no. sc-12726X)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and TFAP2A-DNA complexes were isolated with AP-2α 3B5 antibody (Santa Cruz, catalog no. sc-12726X) .

Sequencing Platform

instrument_model

Illumina HiSeq 3000

hg38

Number of total reads

138020400

Reads aligned (%)

98.2

Duplicates removed (%)

11.3

Number of peaks

3078 (qval < 1E-05)

hg19

Number of total reads

138020400

Reads aligned (%)

97.4

Duplicates removed (%)

13.3

Number of peaks

2459 (qval < 1E-05)