Antigen

Antigen Class

Bisulfite-Seq

Antigen

Bisulfite-Seq

Cell type

Cell type Class

Placenta

Cell type

Placenta

MeSH Description

A highly vascularized mammalian fetal-maternal organ and major site of transport of oxygen, nutrients, and fetal waste products. It includes a fetal portion (CHORIONIC VILLI) derived from TROPHOBLASTS and a maternal portion (DECIDUA) derived from the uterine ENDOMETRIUM. The placenta produces an array of steroid, protein and peptide hormones (PLACENTAL HORMONES).

Sample

source_name

placenta

strain

C57Bl6/J

developmental timepoint

E11.5

genome version

mm10

Sequenced DNA Library

library_strategy

Bisulfite-Seq

library_source

GENOMIC

library_selection

RANDOM

library_construction_protocol

DNA was purified using Qiagen's Puregene kit. MethylC-seq libraries were made using the Epicentre EpiGnome Methyl-Seq kit according to the manufacturer's recommendations except that 14 cycles of amplification were performed.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

73873819

Reads aligned (%)

88.0

Coverage rate (×)

1.4

Number of hyper MRs

247373 (qval < 1E-05)

mm9

Number of total reads

73873819

Reads aligned (%)

88.1

Coverage rate (×)

1.4

Number of hyper MRs

246865 (qval < 1E-05)