GSM4668660: Phase1 cKit high CD25- Runx3 rep2; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
Runx3
Cell type
Cell type Class
Blood
Cell type
T progenitor cells
NA
NA
Attributes by original data submitter
Sample
source_name
BM progenitor-derived T progenitor cells (DNs) in Phase1
treatment
Cultured on OP9DL1
strain
C57BL/6J
developmental stage
cKit high CD25- Phase1 stage
chip antibody
Rabbit anti-Runx3 (provided from Dr. Levanon and Dr. Groner, Levanon et al., 2014)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Zymo ChIP DNA Clean & Concentrator was used to clean eluted DNA after ChIP. For total RNA extraction, the RNeasy Micro Kit (QIAGEN) was used. Sequencing libraries were constructed using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB)