GSM4649146: HCT116: Human colorectal carcinoma cells-RUNX2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
RUNX2
Cell type
Cell type Class
Digestive tract
Cell type
HCT 116
Primary Tissue
Colon
Tissue Diagnosis
Carcinoma
Attributes by original data submitter
Sample
source_name
Human colorectal carcinoma cells
cell line
HCT116
cell type
Human colorectal carcinoma cells
chip antibody
RUNX2(Cell Signaling Technology, #12556)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin immunoprecipitation (ChIP) assays were performed using a ChIP assay kit (Millipore, Temecula, USA) according to the manufacturer's instructions. Briefly, cells were fixed with formaldehyde and homogenized. Nuclei were pelleted, and DNA was sheared by sonication and immunoprecipitated with anti-RUNX2 antibody or negative control immunoglobulin G using protein A/G beads. DNA was released from immunoprecipitate complexes using proteinase K and purified. Chromatin was sheered by 8 sonication cycles to generate DNA fragments with an average size of 200 bp for ChIP-seq analysis and 4 sonication cycles to generate DNA fragments with an average size of 600 bp for ChIP-qPCR. For high-throughput sequencing, 10-20 ng DNA was used to make the ChIP-seq library according to the instructions from Illumina's ChIP-seq Sample Prep kit. The ChIP-seq library was loaded on Illumina's cluster station and sequenced with Illumina Genome Analyzer IIx.