GSM4646925: MDA134-shFOXA1-DOX-WM-E2-ER rep2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
ESR1
Cell type
Cell type Class
Breast
Cell type
MDA-MB-134-VI
Primary Tissue
Breast
Tissue Diagnosis
Carcinoma
Attributes by original data submitter
Sample
source_name
Breast carcinoma cell line
cell line
MDA_MB134 breast carcinoma cell line
agent
Estrogen + DOX
chip antibody
ER (Ab10 Thermo Fisher Scientific, SC-543 Santa cruz)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin was extracted with a sarkosyl based buffer and sonicated to a size range of 200-300 bp DNA sequencing libraries were prepared using the ThruPLEX-FD Prep Kit (Takara Bio USA)