Antigen

Antigen Class

TFs and others

Antigen

ESR1

Cell type

Cell type Class

Breast

Cell type

MDA-MB-134-VI

Primary Tissue

Breast

Tissue Diagnosis

Carcinoma

Sample

source_name

Breast carcinoma cell line

cell line

MDA_MB134 breast carcinoma cell line

agent

Estrogen + DOX

chip antibody

ER (Ab10 Thermo Fisher Scientific, SC-543 Santa cruz)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin was extracted with a sarkosyl based buffer and sonicated to a size range of 200-300 bp DNA sequencing libraries were prepared using the ThruPLEX-FD Prep Kit (Takara Bio USA)

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

17164135

Reads aligned (%)

97.7

Duplicates removed (%)

7.9

Number of peaks

2271 (qval < 1E-05)

hg19

Number of total reads

17164135

Reads aligned (%)

96.8

Duplicates removed (%)

8.2

Number of peaks

2195 (qval < 1E-05)