Antigen

Antigen Class

TFs and others

Antigen

MECOM

Cell type

Cell type Class

Gonad

Cell type

KURAMOCHI

Primary Tissue

Ovary

Tissue Diagnosis

Carcinoma

Sample

source_name

Ovarian cancer cell line

cell type

Ovarian cancer cell line

cell line

Kuramochi

chip antibody

EVI1

antibody catalog

2593

antibody lot

4

young_id

20180817_7452

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Purified immunoprecipitated DNA were prepared for sequencing according to a modified version of the Solexa Genomic DNA protocol. Fragmented DNA was end repaired and subjected to 18 cycles of LM-PCR using oligos provided by Illumina. Amplified fragments between 150 and 300bp (representing shear fragments between 50 and 200nt in length and ~100bp of primer sequence) were isolated by agarose gel electrophoresis and purified. Whole cell extracts were sonicated to solubilize the chromatin. The chromatin extracts containing DNA fragments with an average size of 500 bp were immunoprecipitated using different antibodies.

Sequencing Platform

instrument_model

Illumina HiSeq 4000

hg38

Number of total reads

62889348

Reads aligned (%)

78.7

Duplicates removed (%)

4.6

Number of peaks

7919 (qval < 1E-05)

hg19

Number of total reads

62889348

Reads aligned (%)

78.0

Duplicates removed (%)

5.9

Number of peaks

7986 (qval < 1E-05)