GSM4622849: Patient 1017-11; Homo sapiens; Bisulfite-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Bisulfite-Seq
Antigen
Bisulfite-Seq
Cell type
Cell type Class
Cardiovascular
Cell type
Endomyocardial biopsy
NA
NA
Attributes by original data submitter
Sample
source_name
Patient 1017 Routine EMB
disease state
Heart Transplant Recipient
Sequenced DNA Library
library_strategy
Bisulfite-Seq
library_source
GENOMIC
library_selection
RANDOM
library_construction_protocol
Consented heart transplant recipients provided 8-10 mL of whole blood, which was extracted from the right jugular vein, the same point of entry for the biopsy, and centrifuged at 1900 x g at room temperature for 10 minutes to fractionate the blood. The entire plasma layer was transferred into 2-3 2.0 mL Eppendorf tubes and centrifuged at maximum speed (13,000 RPM) for 16 minutes at 4oC. The resulting supernatant was then used to isolate cell free DNA using the semi-automated MagNA Pure 24 System (Roche).The extracted cell free DNA was then bisulfite coverted using the Epitect Bisulfite Kit (Qiagen). Libraries were prepared using the Illumina MiSeq library preparation kit. Libraries were then sequenced using the v3 MiSeq reagent kit v3 (150 cycles) to produce 2x75 bp paired-end reads