Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
GATA3

Cell type

Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
MCF7-WM-E2-GATA3
cell line
MCF7 breast carcinoma cell line
agent
Estrogen
chip antibody
GATA3 (D13C9, Cell Signaling Technology )

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin was extracted with a sarkosyl based buffer and sonicated to a size range of 200-300 bp DNA sequencing libraries were prepared using the ThruPLEX-FD Prep Kit (Takara Bio USA)

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
23667848
Reads aligned (%)
94.9
Duplicates removed (%)
44.1
Number of peaks
670 (qval < 1E-05)

hg19

Number of total reads
23667848
Reads aligned (%)
94.2
Duplicates removed (%)
45.9
Number of peaks
671 (qval < 1E-05)

Base call quality data from DBCLS SRA