Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
FOXA1

Cell type

Cell type Class
Breast
Cell type
T-47D
Primary Tissue
Breast
Tissue Diagnosis
Adenocarcinoma Ductal

Attributes by original data submitter

Sample

source_name
T47D-WM-E2-FOXA1
cell line
T47D breast carcinoma cell line
agent
Estrogen
chip antibody
FOXA1 (ab5089 Abcam, ab23738 Abcam)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin was extracted with a sarkosyl based buffer and sonicated to a size range of 200-300 bp DNA sequencing libraries were prepared using the ThruPLEX-FD Prep Kit (Takara Bio USA)

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
31646532
Reads aligned (%)
97.7
Duplicates removed (%)
4.9
Number of peaks
17475 (qval < 1E-05)

hg19

Number of total reads
31646532
Reads aligned (%)
96.9
Duplicates removed (%)
6.5
Number of peaks
17278 (qval < 1E-05)

Base call quality data from DBCLS SRA